Interaction of Geminivirus Rep Protein with Replication Factor C and Its Potential Role during Geminivirus DNA Replication

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Geminivirus DNA Replication

Diseases caused by geminiviruses have long been recognized as a limitation to the cultivation of several important crops, including maize, cassava, bean, squash, cucurbits, and tomato, in tropical and subtropical regions of the world. More recently, geminivirus diseases, particularly those transmitted by whiteflies, have become an even greater threat to agriculture due to the appearance of a ne...

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High-frequency reversion of geminivirus replication protein mutants during infection.

The geminivirus replication protein AL1 interacts with retinoblastoma-related protein (RBR), a key regulator of the plant division cell cycle, to induce conditions permissive for viral DNA replication. Previous studies of tomato golden mosaic virus (TGMV) AL1 showed that amino acid L148 in the conserved helix 4 motif is critical for RBR binding. In this work, we examined the effect of an L148V ...

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Dual interaction of a geminivirus replication accessory factor with a viral replication protein and a plant cell cycle regulator.

Geminiviruses replicate their small, single-stranded DNA genomes through double-stranded DNA intermediates in plant nuclei using host replication machinery. Like most dicot-infecting geminiviruses, tomato golden mosaic virus encodes a protein, AL3 or C3, that greatly enhances viral DNA accumulation through an unknown mechanism. Earlier studies showed that AL3 forms oligomers and interacts with ...

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Sequence-specific interaction with the viral AL1 protein identifies a geminivirus DNA replication origin.

The bipartite geminiviruses such as tomato golden mosaic virus (TGMV) and squash leaf curl virus (SqLCV) have two single-stranded circular genomic DNAs, the A and B components, thought to be replicated from double-stranded circular DNA intermediates. Although it has been presumed that the origin sequences for viral replication are located in the highly conserved 200-nucleotide common region (CR...

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Structure and replication of geminivirus genomes.

The geminiviruses are a group of plant viruses containing single-stranded (ss) DNA in particles comprising two quasi-icosahedral units. Some are transmitted by whiteflies, others by leafhoppers. Comparisons were made of the genome organization and expression of cassava latent virus (CLV) and maize streak virus (MSV) and beet curly top virus (BCTV), each with distinct host range and insect vecto...

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ژورنال

عنوان ژورنال: Virology

سال: 2002

ISSN: 0042-6822

DOI: 10.1006/viro.2002.1599